Optimization of Culture Conditions for Maintaining Pig Muscle Stem Cells In Vitro

نویسندگان
چکیده

منابع مشابه

OptimizatiOn Of cOnditiOns Of prOlOnged culture Of pig granulOsa cells in vitro*

the object of the present study was to establish conditions (optimal serum concentration and growth factor supplement) for prolonged culture of pig granulosa cells (gc). gc were cultured in the presence of different fetal calf serum (fcs) concentrations (10% and 20%) and one of the following growth factors: leukemia inhibitory factor (lif), basic fibroblast growth factor (bfgf) and stem cell fa...

متن کامل

Isolation and In vitro Culture of Pig Spermatogonial Stem Cell*

The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for long periods of time in culture. The colonies displayed morphology similar to miceSSC and were positive for markers of SSC (PGP9.5), proliferating germ cell (PigVASA), pre-meiotic germ cell (DAZL) ...

متن کامل

Co-culture of Mouse Embryonic Stem Cells with Sertoli Cells Promote in vitro Generation of Germ Cells

  Objective(s): Sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. The present study was designed to analyze the effect of Sertoli cells in differentiation of mouse embryonic stem cells (mESCs) to germ cells.   Materials and Methods: A fusion construct composed of a Stra8 gene promoter and the coding region of enhanced green fluorescence p...

متن کامل

BETTINA LINDROOS Characterization and Optimization of in Vitro Culture Conditions of Adult Stem Cells for Clinical Cell Therapy

Adipose and dental tissues are attractive and abundant sources of stem cells with therapeutic applicability in diverse medical fields for the repair and regeneration of acute and chronically damaged tissues. Mesenchymal stem cells (MSCs) are found in a variety of tissues including bone marrow, adipose tissue and dental tissues, and reside within the tissue in a hierarchical arrangement maintain...

متن کامل

Optimization of In vitro Culture Conditions of Nasrin (Amaryllis) (Hippeastrum × johnsonii)

Nasrin plant (Amaryllis) is considered as a valuable bulbous plant. The propagation rate of this ornamental plant in natural condition is low. Therefore, application of modern techniques such as tissue culture is an appropriate way to increase the propagation rate of this ornamental plant. In order to study the effect of explant kind, type and concentration of cytokinin on scale explant regener...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Food Science of Animal Resources

سال: 2020

ISSN: 2636-0772,2636-0780

DOI: 10.5851/kosfa.2020.e39